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wild type hela cells  (ATCC)


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    ATCC wild type hela cells
    Wild Type Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hela+wild+type+cells/HeLa/pm41481733-175-0-4
    Average 99 stars, based on 10462 article reviews
    wild type hela cells - by Bioz Stars, 2026-10
    99/100 stars

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    Article Title: High yield purification of an isoleucine zipper-modified CD95 ligand for efficient cell apoptosis initiation and with biotin or DNA-oligomer binding domain to probe ligand functionalization effects
    Article Snippet: The cell lines used in this study were Hela wild type cells and HEK293T cells, which are available from the ATCC organization (Manassas, VA, USA).

    Article Title: High yield purification of an isoleucine zipper-modified CD95 ligand for efficient cell apoptosis initiation and with biotin or DNA-oligomer binding domain to probe ligand functionalization effects.
    Article Snippet: The cell lines used in this study were Hela wild type cells and HEK293T cells, which are available from the ATCC organization (Manassas, VA, USA).

    Article Title: High yield purification of an Isoleucine zipper modified CD95 Ligand with either biotin or DNA-oligomer binding domain for efficient Cell Apoptosis Induction
    Article Snippet: The cell lines used in this study were Hela wild type cells and HEK293T cells, which are available from the ATCC organization (Manassas, VA, USA).

    Modification:

    Article Title: Magnetic Nanoprobes for Spatio-Mechanical Manipulation in Single Cells
    Article Snippet: .. HeLa wild type cells (ATCC ® CCL-2 TM , ATCC, Manassas, VA, USA) were cultivated at 37 °C, 5% CO 2 in Dulbecco’s Modified Eagle Medium (DMEM) medium (Thermo Fisher Scientific) supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific). .. to assess possible negative effects of MNPs on cell viability, a standard CellTiter-Blue (CTB) Assay from Promega (Fitchburg, WI, USA) was carried out for both empty ferritin cages and synomag samples.

    Article Title: Modulation of Efficient Diiodo-BODIPY in vitro Phototoxicity to Cancer Cells by Carbon Nano-Onions
    Article Snippet: .. HeLa wild-type cells (derived from a human cervix carcinoma, ATCC® supplied) were cultured in Dulbecco modified Eagle medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (Life Technologies), 2% penicillin streptomycin (PenStrep) (Life Technologies), and 1% glutamine (Life Technologies) at 37°C in a humidified 5% CO 2 atmosphere. ..

    Derivative Assay:

    Article Title: Modulation of Efficient Diiodo-BODIPY in vitro Phototoxicity to Cancer Cells by Carbon Nano-Onions
    Article Snippet: .. HeLa wild-type cells (derived from a human cervix carcinoma, ATCC® supplied) were cultured in Dulbecco modified Eagle medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (Life Technologies), 2% penicillin streptomycin (PenStrep) (Life Technologies), and 1% glutamine (Life Technologies) at 37°C in a humidified 5% CO 2 atmosphere. ..

    Cell Culture:

    Article Title: Modulation of Efficient Diiodo-BODIPY in vitro Phototoxicity to Cancer Cells by Carbon Nano-Onions
    Article Snippet: .. HeLa wild-type cells (derived from a human cervix carcinoma, ATCC® supplied) were cultured in Dulbecco modified Eagle medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (Life Technologies), 2% penicillin streptomycin (PenStrep) (Life Technologies), and 1% glutamine (Life Technologies) at 37°C in a humidified 5% CO 2 atmosphere. ..



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    Fig. 4 CTB cell viability assay. A. Comparison of His-IZ-CD95L, IZ-CD95L, His-CD95L (Prospec) and Flag-CD95L (Enzo) on <t>HelaWT</t> cell line. Lane 1–4: His- IZ-CD95L at concentration variations 20 ng/ml, 200 ng/ml, 1000 ng/ml, 2000 ng/ml. Lane 5–7: IZ-CD95L at 200 ng/ml, 1000 ng/ml, 2000 ng/ml. Lane 8–11: His-CD95L (Prospec) at 200 ng/ml, 1000 ng/ml, 2000 ng/ml, and His-CD95L cross-linked with 100x α-His mAb. B. Comparison of ligand variants <t>on</t> <t>HEK293T</t> cell line. C. Comparison of ligand variants on MCF7 cell line. D. Comparison of SEC fractions. Biotin-IZ-CD95L fraction peaks A-C collected from SEC analysis (Fig. 3C) and tested at 200 ng/ml. In all conditions: 0.1% Triton X-100 was used as negative control and no treatment as positive controls. Error bars were calculated from three biological replicates. Determination of significance was via one way ANOVA (analysis of variance) test, with * p < 0.05, ** p < 0.01, *** p < 0.001. Detailed results are shown in table S1
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    Fig. 4 CTB cell viability assay. A. Comparison of His-IZ-CD95L, IZ-CD95L, His-CD95L (Prospec) and Flag-CD95L (Enzo) on <t>HelaWT</t> cell line. Lane 1–4: His- IZ-CD95L at concentration variations 20 ng/ml, 200 ng/ml, 1000 ng/ml, 2000 ng/ml. Lane 5–7: IZ-CD95L at 200 ng/ml, 1000 ng/ml, 2000 ng/ml. Lane 8–11: His-CD95L (Prospec) at 200 ng/ml, 1000 ng/ml, 2000 ng/ml, and His-CD95L cross-linked with 100x α-His mAb. B. Comparison of ligand variants <t>on</t> <t>HEK293T</t> cell line. C. Comparison of ligand variants on MCF7 cell line. D. Comparison of SEC fractions. Biotin-IZ-CD95L fraction peaks A-C collected from SEC analysis (Fig. 3C) and tested at 200 ng/ml. In all conditions: 0.1% Triton X-100 was used as negative control and no treatment as positive controls. Error bars were calculated from three biological replicates. Determination of significance was via one way ANOVA (analysis of variance) test, with * p < 0.05, ** p < 0.01, *** p < 0.001. Detailed results are shown in table S1
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    Fig. 4 CTB cell viability assay. A. Comparison of His-IZ-CD95L, IZ-CD95L, His-CD95L (Prospec) and Flag-CD95L (Enzo) on HelaWT cell line. Lane 1–4: His- IZ-CD95L at concentration variations 20 ng/ml, 200 ng/ml, 1000 ng/ml, 2000 ng/ml. Lane 5–7: IZ-CD95L at 200 ng/ml, 1000 ng/ml, 2000 ng/ml. Lane 8–11: His-CD95L (Prospec) at 200 ng/ml, 1000 ng/ml, 2000 ng/ml, and His-CD95L cross-linked with 100x α-His mAb. B. Comparison of ligand variants on HEK293T cell line. C. Comparison of ligand variants on MCF7 cell line. D. Comparison of SEC fractions. Biotin-IZ-CD95L fraction peaks A-C collected from SEC analysis (Fig. 3C) and tested at 200 ng/ml. In all conditions: 0.1% Triton X-100 was used as negative control and no treatment as positive controls. Error bars were calculated from three biological replicates. Determination of significance was via one way ANOVA (analysis of variance) test, with * p < 0.05, ** p < 0.01, *** p < 0.001. Detailed results are shown in table S1

    Journal: BMC biotechnology

    Article Title: High yield purification of an isoleucine zipper-modified CD95 ligand for efficient cell apoptosis initiation and with biotin or DNA-oligomer binding domain to probe ligand functionalization effects.

    doi: 10.1186/s12896-025-00986-2

    Figure Lengend Snippet: Fig. 4 CTB cell viability assay. A. Comparison of His-IZ-CD95L, IZ-CD95L, His-CD95L (Prospec) and Flag-CD95L (Enzo) on HelaWT cell line. Lane 1–4: His- IZ-CD95L at concentration variations 20 ng/ml, 200 ng/ml, 1000 ng/ml, 2000 ng/ml. Lane 5–7: IZ-CD95L at 200 ng/ml, 1000 ng/ml, 2000 ng/ml. Lane 8–11: His-CD95L (Prospec) at 200 ng/ml, 1000 ng/ml, 2000 ng/ml, and His-CD95L cross-linked with 100x α-His mAb. B. Comparison of ligand variants on HEK293T cell line. C. Comparison of ligand variants on MCF7 cell line. D. Comparison of SEC fractions. Biotin-IZ-CD95L fraction peaks A-C collected from SEC analysis (Fig. 3C) and tested at 200 ng/ml. In all conditions: 0.1% Triton X-100 was used as negative control and no treatment as positive controls. Error bars were calculated from three biological replicates. Determination of significance was via one way ANOVA (analysis of variance) test, with * p < 0.05, ** p < 0.01, *** p < 0.001. Detailed results are shown in table S1

    Article Snippet: The cell lines used in this study were Hela wild type cells and HEK293T cells, which are available from the ATCC organization (Manassas, VA, USA).

    Techniques: Viability Assay, Comparison, Concentration Assay, Negative Control